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Poster

Tobacco mosaic virus disks, preparation of samples to investigate mineralization by low voltage electron microscopy

Sunday (01.01.2040)
00:00 - 21:37
Part of:
Line-Up:
- Poster The versatile usage of wrinkled surfaces 1 Bernhard Alexander Glatz
- Poster Penguin feather mimicking oil infused elastomer for anti-icing property 1 Dipl.-Ing. Nguyen Thanh-Binh
- Poster Radiation-based methods for surface modification of supramolecular peptide fibrils 0 Juhaina Bandak
- Poster Investigation of crack propagation of hierarchically structured ceramic materials on different length scales using bending tests 1 Cecilia Müller
- Poster Tobacco mosaic virus disks, preparation of samples to investigate mineralization by low voltage electron microscopy 1 Dr. Sabine Eiben
- Poster Deformation of Liquid-Liquid Phase Boundary as Template for Novel Surface Structured Polymer Particles and Coatings 1 Helena vom Stein
- Poster Sclerenchymatic tissue in Banksia follicles – The effect of moisture on dimensional and mechanical properties 1 Friedrich Reppe
- Poster Reconstructing in-situ nanofibrillar orientation and mechanics in arthropod cuticle using X-ray diffraction modelling 1 Ph.D. Yanhong Wang
- Poster Structural origins of morphing in plant tissues 1 Prof. Dr. Hanoch Daniel Wagner
- Poster What is the effect of cell network topology on the poro-elastic properties of bone? 1 Alexander van Tol
- Poster The fibrillar-level mechanisms of mutability in echinoderm connective tissue analysed using in situ synchrotron small-angle X-ray scattering 1 Dr. Himadri Shikhar Gupta
- Poster Growing bone-like tissues on negative Gaussian curvature surfaces 1 Prof. Dr. John Dunlop
- Poster Gradient porous materials for reliable structured adhesives 1 Ph.D. Di Tan
- Poster Transfer of surface properties of wheat leaves to technical surfaces 1 Miriam Huth
- Poster Damping Behaviour of Bioinspired and Natural Fibre Composites 1 Wilhelm Woigk
- Poster Cancer cells biomineralize ionic gold into nanoparticles-microplates via secreting defense proteins with specific gold-binding peptides 1 Dr. Ajay Vikram Singh
- Poster Microstructural Assessment of Calvarial Fusion in Mice of Different Ages and its Biomechanical Implications 1 Dr. Junning Chen
- Poster Diffusion-limited electroless silver reduction on copper for "porous monocrystal" 1 Dr. Nikolay Ryzhkov
- Poster Multiscale simulations of directed spider dragline silk self-assembly by flow 1 Dipl.-Ing. Ana Herrera
- Poster Inspirational Multiscale Natural Structures 1 Dr. XiaoMeng Sui
- Poster The Exoskeleton of Scorpions: from Structure to Mechanical Function 1 Israel Kellersztein
- Poster Investigating the multilayer fiber-reinforced structure of the wood cell using computer simulations and additive manufacturing 1 Laura Zorzetto
- Poster Light guided 3D-structuring of EPS produced by red algae 1 Moritz Klotz
- Poster Structural optimization of biopolymer composite Cottonid by variation of manufacturing parameters 1 Matthias Langhansl
- Poster Biomineralization of Materials made of Engineered Spider Silk Proteins 1 Vanessa Wicklein
- Poster Micro-Ikebana by Biomimetic Crystallization of Alkaline Earth Carbonates 1 Shota Kobayashi
- Poster Bioinspired hairy surfaces 0 Stefan Müllers
- Poster Reagent-free modification of bio-hydrogels by electron irradiation towards biomedical applications 1 Stefanie Riedel
- Poster Controlled Modification on Wood via SI-ATRP 1 Marta Vidiella del Blanco
- Poster Plant Biomimetics: Surface-Structured Pollen Particles and Transparent Flower Petals 1 Prof. Dr. Olaf Karthaus
- Poster Coupling of polymers to tobacco mosaic virus: towards the production of amphiphilic virus tubes 1 Dr. Sabine Eiben
- Poster Mechanical stable sulfobetainc Hydrogels - A candidate for biomedical application 1 Ramona Bianca Jasmin Ihlenburg
- Poster Structuring V2O5 Nanocomposites to Adapt the Sponge Spicules’ Architecture 1 Achim Diem
- Poster Fibers, Yarns and Non-Woven Meshes – Tough Morphologies Made of Recombinant Spider Silk Proteins 1 Fabian Müller
- Poster Recombinant Spider Silk-based Hybrid Materials for Advanced Energy Technology 1 Tamara Aigner
- Poster Bio-mediated materials syntheses 1 Dr. Daniel Van Opdenbosch
- Poster Design of Biomimetic Thin Coatings Composed of Cellulose Beta-Nanocrystals for Epitaxial Crystallization and Coassembly of 1D Nanoparticles 0 Dipl.-Ing. Zihao Lu
- Poster Seeded Mineralization Leads to Prismatic-Type CaCO3 Thin Coatings with Multiple Structural Functions 0 Ming Li
- Poster Multifunctional Layered Magnetic Composites 1 Christian Debus
- Poster Cuttlebone-inspired Structures for Mechanical Damping 1 Dipl.-Ing. Andrea Knöller

Session P.1: Poster discussion evening
Belongs to:
Session P: Poster Presentation


Tobacco mosaic virus (TMV) has been used as a template for synthesis of nanostructured hybrid functional materials since many years. Through bio-inspired mineralization, a wide range of metal and semiconducting materials e.g SiO2, ZnO, ZnS have been deposited.1, 2. Different TMV coat protein (CP) mutants and a variety of conditions have been applied to study the influence of the biotemplate on the mineralization process. However, studies on the mechanism for deposition of inorganic phases on the biotemplate are scarce. Very recently, we have developed a facile approach for selective deposition of TMV with ZnS, and using conventional optical characterization methods a heterogeneous nucleation was proposed.3 Further, the mineralization of TMV with ZnO nanoparticles with controllable size can be used to extend our explorations in this issue. Since the inner TMV channel is 4 nm in diameter, synthesis of ZnO, which forms bigger crystals already within the mineralization solution, will help to draw conclusions for the deposition mechanism. A possible heterogeneous nucleation in this case could be proven by presence of mineralized material also in the inner virus channel independent of the pre-formed nanoparticles. A possibility to have access to both the outer surface of TMV but also to the inner channel is the use of small artificially produced virus-like particles (VLP) stabilized by a scaffold RNA buried within the protein coat. One of these VLPs are the disks68 (containing 68 CP) with a height of 10 nm. The challenge for investigation by low voltage electron microscopy is to get these disks68 in a pure form without residual free CP or additives like buffer salts. Therefore, we established a purification protocol including size exclusion chromatography which resulted in CP-free disk preparations with a yield of >25%. These disks can now be immobilized horizontally on a surface and be used to investigate the mineralization process.


1. K. Altintoprak, A. Seidenstucker, A. Welle, S. Eiben, P. Atanasova, N. Stitz, A. Plettl, J. Bill, H. Gliemann, H. Jeske, D. Rothenstein, F. Geiger and C. Wege, Beilstein J Nanotech, 2015, 6, 1399-1412.

2. P. Atanasova, N. Stitz, S. Sanctis, J. H. M. Maurer, R. C. Hoffmann, S. Eiben, H. Jeske, J. J. Schneider and J. Bill, Langmuir, 2015, 31, 3897-3903.

3. P. Atanasova, I. Kim, B. Chen, S. Eiben and J. Bill, Advanced Biosystems, 2017, DOI: 10.1002/adbi.201700106, 1700106-n/a.




 

Speaker:
Dr. Sabine Eiben
University of Stuttgart
Additional Authors:
  • Dr. Petia Atanasova
    University of Stuttgart
  • Prof. Dr. Joachim Bill
    University of Stuttgart
  • Dr. Klara Altintoprak
    University of Stuttgart
  • Prof. Dr. Christina Wege
    University of Stuttgart