Short Poster Lecture
Transmission electron microscopy on tobacco mosaic viruses and its heterostructures with ZnOTuesday (20.03.2018) 20:10 - 20:15 Part of:
The aim of the project was to gain insight into bio-inspired mineralization processes of ZnS and ZnO, directed and influenced by the protein coat of tobacco mosaic virus (TMV). The key steps of the mineralization mechanisms were to be evaluated by high-resolution transmission electron microscopy (TEM) imaging of the organic-inorganic interfaces.
The question is tackled from two directions. First, investigating complete multi-layer systems grown with different TMV mutant types regards their structure. Second, following the initial mineral nucleation process on the virus material in the first stages with the help of imaging the statistical positions of the seeds on the virus disks. Here, we want to distinguish between homogeneous and heterogeneous nucleation on the TMV surface.
We show the crucial development of a suitable sample preparation method and optimal imaging conditions. Especially for the virus disk imaging, the sample preparation proved to be very challenging at different stages. Special low-voltage TEM was applied in attempt to minimize electron beam induced sample damage.